Journal: Journal of Inflammation Research

Article Title: Machine Learning Screening and Validation of PANoptosis-Related Gene Signatures in Sepsis

doi: 10.2147/JIR.S461809

Figure Lengend Snippet: Construction of the LPS-induced HUVECs and verification of gene expression. The expression of IL-6 ( A ) and TNF-α ( B ). ( C and D ) The expression of key PANoptosis-related sepsis predictor genes in HUVECs induced by LPS. ns: P ≥ 0.05; *P < 0.05; **P ≤ 0.01; ***p ≤ 0.001; ****P ≤ 0.0001.

Article Snippet: Human Umbilical Vein Endothelial Cells (HUVECs) were used to establish an in vitro model. HUVECs and Dulbecco’s Modified Eagle’s Medium (DMEM) were obtained from Procell Co. Ltd (Wuhan, China).

Techniques: Expressing

Journal: Frontiers in Cardiovascular Medicine

Article Title: Therapeutic Effect of Pericytes for Diabetic Wound Healing

doi: 10.3389/fcvm.2022.868600

Figure Lengend Snippet: The expression of pericyte markers by flow cytometry was reduced in the diabetic condition. (A) Analysis of pericyte surface markers in the non-diabetic and diabetic conditions using flow cytometry [CD146-fluorescein isothiocyanate (FITC), Nestin-phycoerythrin (PE), and NG2-PE]. The results provided here are representative of those obtained in three independent studies. In the diabetic condition, Nestin and NG2-PE were seen to decrease by more than half. (B) Overlay of diabetic condition (green line) and non-diabetic (red line) condition flow cytometry. The diabetic condition showed the three pericyte markers to be decreased.

Article Snippet: Each well contained 300 μl of the human umbilical vein endothelial cells (HUVECs) and pericytes (10:1 ratio) cell suspension in the VascuNet Basal Assay Medium (ESI BIO, USA) ( ).

Techniques: Expressing, Flow Cytometry

Journal: Frontiers in Cardiovascular Medicine

Article Title: Therapeutic Effect of Pericytes for Diabetic Wound Healing

doi: 10.3389/fcvm.2022.868600

Figure Lengend Snippet: The group with pericytes showed improved tube formation and cell migration ability in the high glucose condition. (A) The in-vitro vascular, tube formation assay showed effects of exposure on FITC-tagged human umbilical vein endothelial cell (HUVEC) tube formation with and without PE-tagged human pericyte cells. The optimal observation time for tube formation was 8 h after seeding. In high glucose conditions, HUVECs with pericytes (10:1 ratio) showed active tube formation even compared to the without pericyte group. (B) The number of pieces in the assay was measured. (** P < 0.01 and *** P < 0.001 according to the Student's t -test). HUVEC tube formation capacity showed no significant difference in low glucose conditions. However, in high glucose conditions, the group with pericytes had a statistically significant increase in tube formation. (C) Photomicrographs were taken at 0, 12, and 24 h following initiation of the wound. HUVEC migration, often termed as wound closure ability, was more remarkable in the group with pericytes in both the high and low glucose conditions. (D) The quantitative approach applied to the wound closure assay, showing a histogram calculated based on the group breath ratio of the control group and the group with pericytes.

Article Snippet: Each well contained 300 μl of the human umbilical vein endothelial cells (HUVECs) and pericytes (10:1 ratio) cell suspension in the VascuNet Basal Assay Medium (ESI BIO, USA) ( ).

Techniques: Migration, In Vitro, Tube Formation Assay, Wound Closure Assay, Control

Journal: Frontiers in Cardiovascular Medicine

Article Title: Therapeutic Effect of Pericytes for Diabetic Wound Healing

doi: 10.3389/fcvm.2022.868600

Figure Lengend Snippet: The diabetes mellitus (DM)-with-pericyte group exhibited substantial wound healing in a mouse wound model. (A) Illustration of injection of collagen type 1 scaffold + 5 × 10 5 pericytes in the mouse wound. (B) Streptozotocin (STZ)-induced diabetic mice on blood glucose confirmed successful DM induction (**** P < 0.0001). DM-induced mice ( n = 10), control ( n = 10). (C) Incisions with a diameter of 4 mm on either side of the midline were produced. To avoid wound contraction, the wounds were circumscribed with donut-shaped silicone splints. As observed on day 7, wounds of the DM-with-pericyte group healed, whereas those of the DM-without-pericyte and control groups did not heal. (D) The Y-axis of the graph represents the wound size, when measured on days 0, 2, 7, and 14. It was verified that all the groups, except the DM-without-pericyte group, had wound closure by the time of sacrifice on day 14 and approximately 3.6 mm of the wound of the DM-without-pericyte group remained. The pericyte and DM-with-pericyte groups showed the same wound size on days 0, 2, 7, and 14. (E) Quantification of wound size at sacrifice time points (** P < 0.01). In comparison to the DM-without-pericyte group, the DM-with-pericyte group exhibited substantial wound healing.

Article Snippet: Each well contained 300 μl of the human umbilical vein endothelial cells (HUVECs) and pericytes (10:1 ratio) cell suspension in the VascuNet Basal Assay Medium (ESI BIO, USA) ( ).

Techniques: Injection, Control, Produced, Comparison

Journal: Frontiers in Cardiovascular Medicine

Article Title: Therapeutic Effect of Pericytes for Diabetic Wound Healing

doi: 10.3389/fcvm.2022.868600

Figure Lengend Snippet: The DM-with-pericyte group showed successful repair of the collagen layer and higher blood vessel formation capacity compared to the DM-without-pericyte group. (A) H&E staining. (B) Masson's trichrome staining for wound analysis at 14 days. The black square indicates the regenerated dermal collagen layer. Scale bars = 200 μm. (C) CD31 and Ki67 immunofluorescence of wounds at 14 days. In diabetes wounds, new vessels were significantly less visible than in normal mice. However, when diabetic mice were injected with pericytes, new vessels were observed more often. (D) α-smooth muscle actin (α-SMA), collagen 1 (Col 1), and Nestin immunohistochemistry of wounds at 14 day. Scale bar = 50 μm.

Article Snippet: Each well contained 300 μl of the human umbilical vein endothelial cells (HUVECs) and pericytes (10:1 ratio) cell suspension in the VascuNet Basal Assay Medium (ESI BIO, USA) ( ).

Techniques: Staining, Immunofluorescence, Injection, Immunohistochemistry